Scope of Assays

The table below provides an overview of the scope of each assay available as part of the comprehensive discovery platform which the National Phenome Centre is uniquely able to offer, in addition to the new assays which are currently being developed and validated to come into the Centre in the near future.

Alongside this sit a number of guided monitoring assays. More detailed information regarding the metabolite coverage and volume requirements for each of these assays can be found in the document: MRC-NIHR NPC Assay Portfolio (PDF). Please note that this information is provided for guidance only and we encourage all potential users to contact the Centre to discuss their requirements and needs in more detail.

Assay Matrix Coverage Further information
NMR - broad profiling Urine Broad spectral profile, fundamental to NPC analytical workflow. Assay as defined by NPC covers 3 experiments of differing lengths and methodologies to target different types of analytes. Background publication
RP-LC (POS) - broad profiling mass spec Urine Excellent analyte coverage, from moderately polar to moderately apolar species
RP-LC (NEG) - broad profiling mass spec Urine Complementary analyte coverage, from moderately polar to moderately apolar species
HILIC (POS) - broad profiling mass spec Urine Most similar to NMR in that the targeted compound class is small polar analytes, however provides additional sensitivity Background publication
HILIC (NEG) - broad profiling mass spec Urine Currently under validation - please enquire for further details Background publication
NMR - broad profiling Plasma & Serum Broad spectral profile, fundamental to NPC analytical workflow. Assay as defined by NPC covers 3 experiments of differeing lengths to target different types of analytes.
Lipid screening (POS) - broad profiling mass spec Plasma & Serum Provides in depth coverage of lipid species, complementing NMR
Lipid screening (NEG) - broad profiling mass spec Plasma & Serum Complimentary in depth coverage of lipid species, complimenting NMR
HILIC (POS) - broad profiling mass spec Plasma & Serum Most similar to NMR in that the targeted compound class is small polar analytes, however provides additional sensitivity
HILIC (NEG) - broad profiling mass spec Plasma & Serum Currently under validation - please enquire for further details
RP-LC (POS) - broad profiling mass spec Plasma & Serum Currently under validation - please enquire for further details
RP-LC (NEG) - broad profiling mass spec Plasma & Serum Currently under validation - please enquire for further details
Lipid targeted (NEG) mass spec assay Plasma & Serum Targeted assay for the relative quantification of lipid species Publication on method
Bile acid targeted mass spec assay Urine, Plasma & Serum Targeted assay for the relative quantification of bile acid species. 100 analytes monitored Publication on method
Other targeted mass spec assays Urine, Plasma & Serum Currently under validation - please enquire for further details if you have a specific primary qustion and targeted assay under consideration and would like further advice regarding availability and timeline of assay.