Our workflow has been designed to ensure care of samples received and our analytical platforms provide optimal metabolic coverage . We introduce strict quality control processes through a sample’s analytical journey ensuring high quality, high throughput data.
Stage 1- Formatting
We accept human urine and blood products (serum and plasma) in all different formats.
The volume required depends on the number of assays to be performed.
Please check XXX for recommendations on sample collection.
Upon arrival samples are sorted in a randomised fashion. Our protocol maintain samples frozen at all times.
Samples undergo one single freeze-thaw cycle from the moment they arrive to the centre and when they get analysed and this is when they are formatted into a mother plate.
Stage 2 - Aliquoting
Immediately after, the samples in the mother plate are aliquoted into as many plates as different assays will be performed on the samples.
We also prepare pool samples that are used for quality control.
The aliquoted plates for the different assays are kept at -80 C until the day they are analysed.
Our analytical methods are designed to measure one full plate per day.
Option 1 - Nuclear Magnetic Resonance
We analyse all samples received in the centre firstly by nuclear magnetic resonance spectroscopy (NMR).
NMR is a very robust, reproducible and stable platform that provides a wide coverage of highly abundant small molecules (and some macromolecules).
Option 2 - Mass Spectrometer
We also perform a variety of liquid-chromatography mass spectrometry (LC-MS) assays.
MS is a very sensitive platform that linked to different types of LC provide a wide coverage of small molecules and lipids.